Bachelor Student

Polycomb response elements (PREs) in Drosophila are well- characterised, but knowledge about mammalian PREs is lacking. In order to fill this gap, we have used computational prediction of mammalian PREs, followed by high throughput testing in mouse cell culture to evaluate the properties of these predicted PREs. Before this validation the aim of my bachelors project is to improve the efficiency of method for generating the constructs to be tested. I have established a new primer design for this step. Constructs are generated by Gibson Assembly and correct assembly is evaluated after transformation into E. coli. Successful transformation of the essential plasmid is also proof that the new primers are working correctly. With this method the process of high throughput validation can be streamlined, save costs and eventually will contribute to a long standing question: what makes a mammalian PRE?